Determination of chyodeoxycholic acid in Bile Arisaema by HPLC-ELSD / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 1634-1636, 2007.
Article
Dans Chinois
| WPRIM
| ID: wpr-287879
ABSTRACT
<p><b>OBJECTIVE</b>To develop an HPLC method for the determination of hyodeoxycholic acid in Bile Arisaema.</p><p><b>METHOD</b>The methanol extracts were separated on a Diamonsil ODS column eluted at 35 degrees C with a mixture of methanol-water-glacial acetic acid (75250.01) at flow-rate 1.0 mL x min(-1). The drift-tube temperature of ELSD was 85 degrees C, and the flow rate of air was 3.0 L x min(-1).</p><p><b>RESULT</b>Hyodeoxycholic acid in methanol extract was well separated, the relationship of logarithms of injection amount and peak area was linear (r = 0.9998) over the range of 2.4-24 microg. The average recovery was 97.1% (RSD 2.9%, n=5).</p><p><b>CONCLUSION</b>The method could be used for quality control of Bile Arisaema.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Plantes médicinales
/
Contrôle de qualité
/
Normes de référence
/
Diffusion de rayonnements
/
Bile
/
Chimie
/
Reproductibilité des résultats
/
Chromatographie en phase liquide à haute performance
/
Arisaema
/
Tubercules
Type d'étude:
Etude diagnostique
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
China Journal of Chinese Materia Medica
Année:
2007
Type:
Article
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