Expression and significance of GCS gene in human pancreatic cancer cell SW1990 and its drug-resistant sublines / 中华外科杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the expression and significance of GCS gene in human pancreatic cancer cell line SW1990 and its drug-resistant sublines.</p><p><b>METHODS</b>SW1990 and its drug-resistant sublines, SW1990/FU, SW1990/ADM and SW1990/GEM were cultured in vitro. CCK-8 (Cell Counting kit-8) was used to detect the drug resistance of the sublines. Relative quantitation of GCS mRNA expression was evaluated by real-time PCR and Western blot was adopted to evaluate the expression of GCS protein.</p><p><b>RESULTS</b>The drug resistance indexes of SW1990/FU, SW1990/ADM and SW1990/GEM were 339.7, 11.9 and 56.6, respectively. The GCS mRNA and protein were expressed in all SW1990 and its drug-resistant sublines. There was a higher expression of GCS mRNA in all the sublines and a significant difference of GCS protein expression was detected in SW1990/ADM and SW1990/GEM compared with SW1990.</p><p><b>CONCLUSIONS</b>GCS gene is expressed in SW1990 and its drug-resistance sublines. The high expression of GCS protein in SW1990/ADM and SW1990/GEM might be one reason of resistance to ADM and GEM in the sublines.</p>