Detection of PTCH gene mutations in odontogenic keratocysts by SSCP and DNA sequencing / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 293-296, 2006.
Article
Dans Chinois
| WPRIM
| ID: wpr-288947
ABSTRACT
<p><b>OBJECTIVE</b>To investigate PTCH gene mutations in odontogenic keratocysts (OKC).</p><p><b>METHODS</b>PCR-SSCP and DNA sequencing were used to analyze the PTCH gene mutations in 12 OKCs, including 10 sporadic and 2 nevoid basal cell carcinoma syndrome (NBCCS) associated OKC.</p><p><b>RESULTS</b>Four mutations were identified in 4 cysts, among which two germline mutations were associated with NBCCS and 2 somatic mutations were in 2 unrelated sporadic cases. In addition, eight previously reported polymorphisms in the PTCH gene were also found in 10 cases.</p><p><b>CONCLUSION</b>The present study indicated that both sporadic and NBCCS-related OKCs could carry PTCH gene mutation. Thus, mutational inactivation of PTCH gene may play a significant role in the pathogenesis of OKC.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Polymorphisme génétique
/
Naevomatose basocellulaire
/
Kystes odontogènes
/
Réaction de polymérisation en chaîne
/
Analyse de séquence d'ADN
/
Récepteurs de surface cellulaire
/
Polymorphisme de conformation simple brin
/
Mutation
Type d'étude:
Etude diagnostique
Limites du sujet:
Femelle
/
Humains
langue:
Chinois
Texte intégral:
West China Journal of Stomatology
Année:
2006
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS