Establishment of autologous endometrial coculture and sequential system for human early embryo culture / 中华男科学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish a coculture and a sequential system for human early embryo culture.</p><p><b>METHODS</b>The endometrial tissue was digested enzymatically and cultured to achieve generated and cryo-thawed endometrial monolayer cells. The generated and cryo-thawed monolayer cells were cocultured with human 2PN embryos and transferred to sequential medium every 48 hours.</p><p><b>RESULTS</b>Human endometrial cells had viability in vitro culture. The autologously generated and cryo-thawed monolayer cells were successfully obtained, and 74.04% of the cryo-thawed cells were successfully used in coculturing human early embryos. The embryos developed well, with the clinical pregnancy rate of 68.83% and the implantation rate of 44.23%.</p><p><b>CONCLUSION</b>The autologous endometrial cell coculture and sequential culture system for human early embryo development provides a feasible method for studying human embryo development and implantation so as to improve embryo quality.</p>