Inhibitory effect of SHP-1 gene transfer on the proliferation of breast cancer cell line MDA-MB-231 / 南方医科大学学报
Journal of Southern Medical University
; (12): 1024-1027, 2010.
Article
Dans Zh
| WPRIM
| ID: wpr-290003
Responsable en Bibliothèque :
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe SHP-1 protein expression in breast cancer cell line MDA-MB-231 before and after SHP-1 gene transfer and its effect on the proliferation of MDA-MB-231 cells.</p><p><b>METHODS</b>The eukaryotic expression vector pEGFP-C3-SHP-1 was constructed and transfected into breast cancer cell line MDA-MB-231 via Lipofectamine 2000, and the positive clones were selected using G418. SHP-1 expression in MDA-MB-231 cells was detected with immunocytochemistry and Western blotting, and the cell growth curve was observed using MTT assay.</p><p><b>RESULTS</b>SHP-1 was highly expressed in transfected MDA-MB-231 cells, whose proliferation was significantly inhibited (P<0.05).</p><p><b>CONCLUSION</b>SHP-1 gene transfer into MDA-MB-231 cells results in inhibition of the cell proliferation.</p>
Texte intégral:
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Indice:
WPRIM
Sujet Principal:
Anatomopathologie
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Tumeurs du sein
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Techniques de transfert de gènes
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Lignée cellulaire tumorale
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Prolifération cellulaire
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Protein Tyrosine Phosphatase, Non-Receptor Type 6
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Génétique
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Métabolisme
Limites du sujet:
Female
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Humans
langue:
Zh
Texte intégral:
Journal of Southern Medical University
Année:
2010
Type:
Article