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The role of mir-221/222 in inhibiting endoplasmic reticulum stress-induced human hepatocarcinoma cell apoptosis / 中华肝脏病杂志
Chinese Journal of Hepatology ; (12): 191-195, 2011.
Article Dans Chinois | WPRIM | ID: wpr-290605
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the role of miR-221/222 in inhibiting endoplasmic reticulum stress-induced human hepatocarcinoma cells apoptosis.</p><p><b>METHOD</b>miR-221/222 mimics and inhibitors were used to mimic or block the function of endogenous miR-221/222 respectively. Western blot and flow cytometry were used to test the effects of miR-221/222 on cell cycle and apoptosis under endoplasmic reticulum stress in human hepatocellular carcinoma cells.</p><p><b>RESULTS</b>Endoplasmic reticulum stress resulted in miR-221/222 down-regulation in human hepatocellular carcinoma cells. miR-221/222 mimics and inhibitors inhibited and promoted respectively endoplasmic reticulum stress-mediated p27Kip1 induction. Moreover, p27Kip1 suppression not only resulted in reduction in the fraction of G1 phase cells, but also promoted the endoplasmic reticulum stress-mediated apoptosis in human hepatocellular carcinoma cells.</p><p><b>CONCLUSION</b>miR-221/222 were downregulated by endoplasmic reticulum stress in human hepatocellular carcinoma cells, which subsequently protected human hepatocellular carcinoma cells against endoplasmic reticulum stress-induced apoptosis through p27Kip1 regulation.</p>
Sujets)
Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Anatomopathologie / Cycle cellulaire / Apoptose / Carcinome hépatocellulaire / MicroARN / Lignée cellulaire tumorale / Réticulum endoplasmique / Inhibiteur p27 de kinase cycline-dépendante / Tumeurs du foie / Métabolisme Limites du sujet: Humains langue: Chinois Texte intégral: Chinese Journal of Hepatology Année: 2011 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Anatomopathologie / Cycle cellulaire / Apoptose / Carcinome hépatocellulaire / MicroARN / Lignée cellulaire tumorale / Réticulum endoplasmique / Inhibiteur p27 de kinase cycline-dépendante / Tumeurs du foie / Métabolisme Limites du sujet: Humains langue: Chinois Texte intégral: Chinese Journal of Hepatology Année: 2011 Type: Article