Protective effect of isoorientin on alcohol-induced hepatic fibrosis in rats / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 3726-3730, 2013.
Article
Dans Chinois
| WPRIM
| ID: wpr-291295
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effect and mechanism of isoorientin from Gypsophila elegans on alcohol-induced hepatic fibrosis in rats.</p><p><b>METHOD</b>ninety healthy male Wistar rats were randomly divided into six groups the normal control group, the model control group, the colchicines group (positive control, 1.0 mg x kg(-1) x d(-1)), the high, middle and low-dose isoorientin groups (20, 50, 100 mg x kg(-1) x d(-1)). The normal control group received normal saline, while other groups received alcohol to cause hepatic fibrosis. After 24-weeks treatment, the alanine aminotransferase (ALT), aspartate aminotransferase (AST), Interleukin 6 (IL-6), tumor necrosis factor-alpha (TNF-alpha), hyaluronic acid (HA), laminin (LN), type III precollagen (PCIII), hydroxyproline (Hyp), Myeloperoxidase (MPO), malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) were assayed according to the manufacturer's instructions, the alpha-SMA and TGF-beta1 were detected by western blotting, and the histopathological changes was observed by H&E staining.</p><p><b>RESULT</b>Isoorientin could improve the liver function by decreasing the activity of ALT, AST, IL-6, TNF-alpha, MDA, MPO, HA, LN, PCIII and Hyp (P < 0.05), increasing the activity of SOD and GSH-Px (P < 0.05), and reducing the expression of alpha-SMA and TGF-beta1 (P < 0.05). In addition, the high and middle-dose isoorientin groups showed more remarkable effect</p><p><b>CONCLUSION</b>Isoorientin from G. elegans can protect hepatic fibrosis induced by alcohol.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Aspartate aminotransferases
/
Sang
/
Médicaments issus de plantes chinoises
/
Rat Wistar
/
Agents protecteurs
/
Lutéoline
/
Alanine transaminase
/
Éthanol
/
Traitement médicamenteux
/
Facteur de croissance transformant bêta-1
Type d'étude:
Étude pronostique
Limites du sujet:
Animaux
/
Humains
/
Mâle
langue:
Chinois
Texte intégral:
China Journal of Chinese Materia Medica
Année:
2013
Type:
Article
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