Effects of 2A-1-1 on the aggregation and Ca2+ influx of platelets / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 544-547, 2004.
Article
Dans Chinois
| WPRIM
| ID: wpr-291382
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effects of 2A-1-1 (purified component from Panax notoginsengs saponins) on the aggregation of and Ca2+ influx into human platelets.</p><p><b>METHODS</b>The aggregation of platelets was tested by nephelometry, Fura-2 fluorescent technique was used for detecting cell [Ca2+]i. The effects of 2A-1-1, nifedipine and SK&F96365 on Ca(2+) influx into human platelets induced by ADP or CPA were observed separately.</p><p><b>RESULTS</b>Nifedipine (< 20 micromol/L) could not inhibit platelet aggregation induced by ADP or the Ca(2+) influx induced by ADP or CPA. SK&F96365 at 20 micromol/L could inhibit the maximal aggregation of platelets induced by ADP with a inhibitory rate of 59.83%, at 15 micromol/L could inhibit the Ca2+ influx induced by CPA or ADP. 2A-1-1 (5, 10 and 20 micromol/L) could inhibit the maximal aggregation of platelets induced by ADP with the inhibitory rates of 47.06%, 53.47% and 71.52%, respectively. 2A-1-1 at 10 and 20 micromol/L could inhibit the Ca2+ influx induced by CPA or ADP.</p><p><b>CONCLUSIONS</b>2A-1-1 can inhibit platelets aggregation, block the ROC (Receptor-dependent Ca2+ channels) and inhibit Ca2+ influx of human platelets.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pharmacologie
/
Plaquettes
/
Pharmacocinétique
/
Antiagrégants plaquettaires
/
Inhibiteurs des canaux calciques
/
Nifédipine
/
ADP
/
Agrégation plaquettaire
/
Calcium
/
Biologie cellulaire
Limites du sujet:
Adulte
/
Femelle
/
Humains
/
Mâle
langue:
Chinois
Texte intégral:
Chinese Journal of Hematology
Année:
2004
Type:
Article
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