Study of HLA polymorphism in the 6965 Han bone marrow registry donors / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 473-477, 2004.
Article
Dans Chinois
| WPRIM
| ID: wpr-291394
ABSTRACT
<p><b>OBJECTIVE</b>To analyze human leukocyte antigen (HLA) polymorphism and search for new alleles in Chinese Han population bone marrow registry donors.</p><p><b>METHODS</b>DNA-based HLA genotyping methods were used including PCR-SSP, BST and molecular cloning.</p><p><b>RESULTS</b>A total of 6965 unrelated donors, 4707 from South China origin and 2258 from north, were typed for HLA-A, B, and DRB1 loci. Seventy-two specificities of HLA alleles were identified. The HLA-A25, A34, A74, B41, B42, B53, B73 and B81 that were rarely reported in previously Chinese population studies were identified in this study. Estimation of gene frequency indicated that the blank gene frequency was less than 0.2% for HLA-A, 0.25% for HLA-B and 0.70% for HLA-DRB1 loci. Three novel alleles were identified and officially assigned by the World Health Organization (WHO) Nomenclature Committee as A*0253N, A*1114 and B*5610.</p><p><b>CONCLUSION</b>Large-scale DNA-based HLA genotyping used in bone marrow registry donors is highly accurate and reliable for estimating gene frequency and searching for new alleles. The discrepancy of HLA gene distribution between South and North China Han population showed the necessity of setting the more regions in South and North China to screen the bone marrow registry donors for bone marrow transplant.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Polymorphisme génétique
/
Donneurs de tissus
/
Antigènes d'histocompatibilité de classe I
/
Antigènes HLA-A
/
Antigènes HLA-B
/
Antigènes HLA-DR
/
Antigènes d'histocompatibilité de classe II
/
Chine
/
Enregistrements
/
Transplantation de moelle osseuse
Limites du sujet:
Femelle
/
Humains
/
Mâle
Pays comme sujet:
Asie
langue:
Chinois
Texte intégral:
Chinese Journal of Hematology
Année:
2004
Type:
Article
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