Activating effects of protein transduction domain mediated BCR/ABL protein on CML T cells / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 644-647, 2003.
Article
Dans Chinois
| WPRIM
| ID: wpr-291477
ABSTRACT
<p><b>OBJECTIVE</b>To study the activating effect of protein transduction domain (PTD) mediated BCR/ABL protein on T cells from CML patients.</p><p><b>METHODS</b>The plasmid containing PTD and b3a2 bcr/abl of CML was constructed by genetic engineering and expressed in E. coli. The peripheral blood mononuclear cells from CML patients were stimulated in vitro with purified PTD-BCR/ABL protein and the expression of the early activation antigen CD(69) on CD(8)(+) and CD(4)(+) T cells was detected by flow cytometry (FCM).</p><p><b>RESULTS</b>The optimal concentration of PTD-BCR/ABL protein for activating CD(8)(+) T cells in vitro was 100 micro g/ml, CD(69) expression peaked in three days stimulation. CD(8)(+) T cells were activated in 10 of 15 CML patients, the expression rate of CD(69) was (15.01 +/- 3.75)%. CD(4)(+) T cells were activated in 4 of 15 patients, the expression rate of CD(69) was (10.32 +/- 3.08)%. Both CD(8)(+) and CD(4)(+) T cells were activated simultaneously in 3 of them. However, neither CD(4)(+) nor CD(8)(+) T cells was activated by stimulation with BCR/ABL protein in all 15 specimens, the expression rate of CD(69) on CD(8)(+) and CD(4)(+) T cells was (1.36 +/- 0.31)% and (1.41 +/- 0.43)%, respectively. There was no difference compared with that of PBS control group (P > 0.05).</p><p><b>CONCLUSION</b>By using a PTD-mediated antigen delivering system, exogenous BCR/ABL protein can be delivered into APC, processed and presented onto surface of APC to activate Ag-specific CD(8)(+) and CD(4)(+) T cells in vitro.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pharmacologie
/
Sang
/
Protéines de fusion recombinantes
/
Lymphocytes T
/
Leucémie myéloïde chronique BCR-ABL positive
/
Antigènes de différenciation des lymphocytes T
/
Antigènes CD
/
Antigènes CD4
/
Protéines du gène tat
/
Protéines de fusion bcr-abl
Limites du sujet:
Adolescent
/
Adulte
/
Adulte très âgé
/
Femelle
/
Humains
/
Mâle
langue:
Chinois
Texte intégral:
Chinese Journal of Hematology
Année:
2003
Type:
Article
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