The changes of gene expression of iron transporters for duodenal iron uptake and export in diet-induced obese mice / 中华预防医学杂志
Chinese Journal of Preventive Medicine
;
(12): 275-278, 2015.
Article
Dans Chinois
| WPRIM
| ID: wpr-291601
ABSTRACT
<p><b>OBJECTIVE</b>This study aims to determine the gene expression changes of iron transporters-divalent metal transporter 1 (DMT1) and ferroportin 1 (Fpn1) in the duodenal tissue of diet-induced obese mice.</p><p><b>METHODS</b>C57BL/6J mice were randomly divided into normal control (NC) and obesity model (OM) group, 6 in each, and fed on conventional and high-fat diet respectively for 14 weeks by table of random number. Then the DMT1 and Fpn1 mRNA contents in duodenal tissues of the animals were measured by Real-time PCR method, and the protein expression levels were analyzed by Western blot test.</p><p><b>RESULTS</b>The Real-time PCR detection results showed that, compared with the NC group for which the mRNA expression level was defined as 1.0, the Fpn1 mRNA expression in OM group (0.58±0.11) was reduced significantly (t = 6.71, P = 0.014), whereas the relative expression level of DMT1 mRNA in OM group (0.89±0.26) showed no obvious alteration (t = 2.01, P = 0.122). Western blot results showed that the relative protein expression levels of Fpn1 in OM and NC group were 0.32±0.06 and 0.65±0.19, respectively, and the difference was statistically significant (t = 5.37, P = 0.026). The DMT1 protein relative abundance was 0.88±0.21 in OM group and 0.92±0.17 in NC group, and the difference has no statistical significance (t = 1.84, P = 0.185).</p><p><b>CONCLUSION</b>Fpn1 gene expression is inhibited in the duodenum of diet-induced obesity mouse while DMT1 expression keeps unchanged, and this implies that decreased iron export from enterocytes into circulation might be responsible for the impaired iron absorption in obesity.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
ARN messager
/
Expression des gènes
/
Transporteurs de cations
/
Régime alimentaire
/
Duodénum
/
Alimentation riche en graisse
/
Fer
/
Souris de lignée C57BL
/
Souris obèse
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Chinese Journal of Preventive Medicine
Année:
2015
Type:
Article
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