Identification of a novel HLA allele HLA-DRB1*09:01:07 / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 663-665, 2014.
Article
Dans Chinois
| WPRIM
| ID: wpr-291706
ABSTRACT
<p><b>OBJECTIVE</b>To identify and confirm a novel HLA allele in a Chinese Han individual.</p><p><b>METHODS</b>HLA typing was performed by polymerase chain reaction-sequence specific oligonucleotide probes (PCR-SSOP) for HLA-A, -B and -DRB1 in a registered donor of China Marrow Donor Program(CMDP). Sequencing-based typing (SBT) was carried out to further confirm the novel allele of HLA-DRB1.</p><p><b>RESULTS</b>The SSOP result showed HLA-DRB1*0903,15GEP, but an unusual pattern that could not be defined indicated potential presence of a novel allele. The SBT result showed the novel sequence has 1nt change from its closest allele DRB1*090102 at nt306 where C to T(codon 73 GCC to GCT) resulting in no amino acid change. 73A was not changed.</p><p><b>CONCLUSION</b>A novel HLA allele, HLA-DRB1*090107, has been identified and named officially by WHO Nomenclature Committee for Factors of the HLA System.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Donneurs de sang
/
Test d'histocompatibilité
/
Données de séquences moléculaires
/
Séquence nucléotidique
/
Réaction de polymérisation en chaîne
/
Analyse de séquence d'ADN
/
Polymorphisme de conformation simple brin
/
Chaines HLA-DRB1
/
Génétique
/
Méthodes
Type d'étude:
Étude pronostique
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Medical Genetics
Année:
2014
Type:
Article
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