Effects of millimeter wave on gap junctional intercellular communication in human keratinocytes / 中华预防医学杂志
Chinese Journal of Preventive Medicine
; (12): 8-10, 2004.
Article
Dans Zh
| WPRIM
| ID: wpr-291825
Responsable en Bibliothèque :
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effect of millimeter wave (MW) at low power density on gap junctional intercellular communication (GJIC) in human keratinocytes (HaCaTs).</p><p><b>METHODS</b>Fluorescence recovery after photobleaching (FRAP) technique was employed to determine effect of 30.16 GHz MW exposure at 1.0 and 3.5 mW/cm(2) on GJIC with laser confocal scanning microscope.</p><p><b>RESULTS</b>FRAP analysis revealed that 12-O-tetradecanoylphorbol-13-acetate (TPA) at a dose of 5 microg/L could inhibit GJIC in HaCaTs. Fluorescence recovery rate fell from (55 +/- 17)% in the controls to (34 +/- 13)% after photobleaching, with a very significant difference (P < 0.001). Exposure to MW alone for one hour at either 1.0 mW/cm(2) or 3.5 mW/cm(2) did not affect GJIC, with fluorescence recovery rates of (52 +/- 16)% and (50 +/- 17)%, respectively. GJIC suppression induced by TPA was weakened by MW combined with 5 microg/L TPA treatment for one hour, which could be partially recovered by exposure to 1.0 mW/cm(2) MW with fluorescence recovery rate of (47 +/- 16)%, P < 0.01, and fully recovered by exposure to 3.5 mW/cm(2) MW with fluorescence recovery rate of (50 +/- 16)%, P < 0.001, with a very significant difference.</p><p><b>CONCLUSIONS</b>GJIC suppression induced by TPA could be eliminated or diminished by exposure to millimeter wave in HaCaTs.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Pharmacologie
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Physiologie
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Effets des rayonnements
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12-Myristate-13-acétate de phorbol
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Kératinocytes
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Communication cellulaire
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Lignée cellulaire
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Jonctions communicantes
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Biologie cellulaire
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Redistribution de fluorescence après photoblanchiment
Limites du sujet:
Humans
langue:
Zh
Texte intégral:
Chinese Journal of Preventive Medicine
Année:
2004
Type:
Article