Establishment of human embryonic stem cell line NJGLLhES1 / 中华男科学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To report the derivation and characterization of a new human embryonic stem cell (hESC) line NJGLLhES1.</p><p><b>METHODS</b>From the inner cell mass of frozen-thawed human embryos and with ICR mouse embryonic fibroblasts as the feeder layer, we established a new human embryonic stem cell line, which was named NJGLLhES1. We detected the karyotype of the cell line, determined the expressions of alkaline phosphatase, the specific cell surface antigens SSEA-3, SSEA-4, TRA-1-60, TRA-1-81 and the marker gene Oct-4, and examined the formation of embryoids and teratomas.</p><p><b>RESULTS</b>NJGLLhES1 was maintained for over 1 year in vitro, with the morphological characteristics of hESC, a normal karyotype, positive expressions of alkaline phosphatase and specific cell marker genes, and the potential of forming embryoids and teratomas.</p><p><b>CONCLUSION</b>A new human embryonic stem cell line NJGLLhES1 was successfully established, which remains karyotypically and phenotypically stable, undifferentiated and capable of self-renewal and pluripotential differentiation.</p>