In vitro study of human multiple myeloma cell transfected with soluble vascular endothelial growth factor receptor-1 gene / 癌症
Chinese Journal of Cancer
;
(12): 65-68, 2010.
Article
Dans Chinois
| WPRIM
| ID: wpr-292638
ABSTRACT
<p><b>BACKGROUND AND OBJECTIVE</b>Vascular endothelial growth factor (VEGF) is the most important angiogenic factor of multiple myeloma (MM). This study was to investigate the effect of transfection of human soluble vascular endothelial growth factor receptor-1 (sFlt-1) gene on the proliferation of human MM cell line RPMI8226.</p><p><b>METHODS</b>The recombinant plasmid pcDNA3-sFlt-1 was constructed and transfected into RPMI8226 cells. The expression of sFlt-1 was identified by reverse transcription-polymerase chain reaction (RT-PCR) and ELISA. The effects of sFlt-1 protein on the proliferation and VEGF expression of RPMI8226 cells were investigated by MTT assay and ELISA, respectively.</p><p><b>RESULTS</b>The recombinant plasmid pcDNA3-sFlt-1 was successfully transfected into RPMI8226 cells. sFlt-1 protein expression was identified by ELISA, which inhibited the proliferation of RPMI8226 cells and reduced VEGF concentration in the culture supernatant.</p><p><b>CONCLUSION</b>RPMI8226 cells can express sFlt-1 protein with high biological activity when transfected with the sFlt-1 gene, which inhibits the proliferation of RPMI8226 cells.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
Physiologie
/
Plasmides
/
Protéines recombinantes
/
Test ELISA
/
Transfection
/
Régulation de l'expression des gènes tumoraux
/
RT-PCR
/
Récepteur-1 au facteur croissance endothéliale vasculaire
/
Lignée cellulaire tumorale
Type d'étude:
Étude pronostique
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Cancer
Année:
2010
Type:
Article
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