The effect of transforming growth factor-beta1 on EDA region of fibronectin in oral squamous cell carcinoma and adenoid cystic carcinoma cells / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 47-51, 2007.
Article
Dans Chinois
| WPRIM
| ID: wpr-292988
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of transforming growth factor-beta1 (TGF-beta1) on EDA region of fibronectin in oral squamous cell carcinoma and adenoid cystic carcinoma cells.</p><p><b>METHODS</b>Immunohistochemistry and reverse transcription polymerase chain reaction technique were used to detect the changes of EDA proteins and mRNAs expression.</p><p><b>RESULTS</b>The immunostaining ratio in Tca83 cells with TGF-beta1 was greatly higher than that in Tca83 cells without TGF-beta1 (P < 0.01), the immunostaining ratio in SACC-83 cells with TGF-beta1 was higher than that in SACC-83 cells without TGF-beta1 (P < 0.05), but there was no significant difference between SACC-LM cells with TGF-beta1 and SACC-LM cells without TGF-beta1 (P > 0.05). In the three oral carcinoma cells, the expression of EDA(+) mRNAs in the group with TGF-beta1 was higher than that in the group without TGF-beta1 (P < 0.05). The expression of EDA(-) mRNAs in the group with TGF-beta1 was lower than that in the group without TGF-beta1 (P < 0.05).</p><p><b>CONCLUSIONS</b>TGF-beta1 could influence EDA region splicings and upregulate the expression of EDA region in Tca83, SACC-83 and SACC-LM cells, and might play an important role in accelerating oral carcinoma cells adhesion and tumor invasion and metastasis.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
Pharmacologie
/
ARN messager
/
Tumeurs de la bouche
/
Carcinome épidermoïde
/
Fibronectines
/
Carcinome adénoïde kystique
/
Lignée cellulaire tumorale
/
Facteur de croissance transformant bêta-1
/
Métabolisme
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Stomatology
Année:
2007
Type:
Article
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