Different expression of cytokines induced by Actinobacillus actinomycetemcomitans lipopolysaccharide in monocytes/macrophages from different organs of rabbits / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 155-160, 2013.
Article
Dans Chinois
| WPRIM
| ID: wpr-293634
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of cytokines induced by Actinobacillus actinomycetemcomitans lipopolysaccharide (Aa-LPS) in monocytes/macrophages from different organs of rabbits.</p><p><b>METHODS</b>The peripheral mononuclear cells (Mo), alveolar macrophages (AM), peritoneal macrophages (PM) and Kupffer cells (KC) from five New Zealand rabbits were isolated respectively. Then the cells from different organs were stimulated with Escherichia coli (Ec)-LPS or Aa-LPS at the dose of 1 mg/L. After culture for 24 hours, the expression of tumor necrosis factor-α (TNF-α), interleukin (IL)6, IL-1β, IL-8 mRNA and protein were determined by real-time PCR and enzyme-linked immunosorbent assay respectively.</p><p><b>RESULTS</b>The monocytes/macrophages challenged by Ec-LPS or Aa-LPS expressed more cytokines both in mRNA and protein levels compared with the controls (P < 0.05). Among them, AM displayed the highest respond when encount with Aa-LPS, with the TNF-α, IL-6, IL-1β, IL-8 mRNA relative levels were (0.4719 ± 0.0171), (2.7895 ± 0.0669), (5.1527 ± 0.1190), (3.6785 ± 0.1836) and the proteins concentrations were (82.2 ± 5.4), (40.2 ± 2.0), (50 308.3 ± 445.0), (35 305.3 ± 1480.9) ng/L respectively. And the inducibility of Aa-LPS was stronger than that of Ec-LPS (P < 0.05). Meanwhile the cells from different organs showed discrepant response when exposed to Aa-LPS (P < 0.05). The results showed their abilities to secrete cytokines were in the sequence of AM > Mo > KC > PM.</p><p><b>CONCLUSIONS</b>Aa-LPS influenced the expression of cytokines in monocytes/macrophages from different organs of rabbits.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
ARN messager
/
Monocytes
/
Cellules cultivées
/
Lipopolysaccharides
/
Interleukine-8
/
Interleukine-6
/
Facteur de nécrose tumorale alpha
/
Macrophages alvéolaires
/
Aggregatibacter actinomycetemcomitans
/
Macrophages péritonéaux
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Chinese Journal of Stomatology
Année:
2013
Type:
Article
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