Study on ligustrazine in reversing multidrug resistance of HepG2/ADM cell in vitro / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the reverse effect of ligustrazine (TMP) on HepG2/ADM, a herd of hepatocellular carcinoma cell, multidrug resistance (MDR) and the influence of P-gp170 expression.</p><p><b>METHOD</b>The reverse effect of ligustrazine on HepG2/ADM cell was observed, with the methods of cell culture, MTT's analyze, RT-PCR and Flow cytometric, etc.</p><p><b>RESULT</b>Ligustrazine could make MDR of cell line of HepG2/ADM reduce the expression of P-gp170, enhance the density of adriamycin in cell and increase the adriamycin's cytotoxicity. With the Flow cytometric, the results of RT-PCR showed the transcriptional activity of the MDR1 decreased.</p><p><b>CONCLUSION</b>Ligustrazine can reverse MDR of HCC cell line of HepG2/ADM and has prospect in clinical use.</p>