Determination of epimedin C and icariin in Herba Epimedii by HPLC / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish a RP-HPLC method for the determination of epimedin C and icariin in Herba Epimedii.</p><p><b>METHOD</b>Chromatographic conditions Hypersil BDS-C18 column, acetonitrice and water containing 0.05% phosphoric acid as gradient eluents, G1315A photodiode array detector at 272 nm.</p><p><b>RESULT</b>The average recovery rate of epimedin C was 99.7%, RSD 1.5% (n = 9), icariin 102.5%, RSD 1.1% (n=9).</p><p><b>CONCLUSION</b>The method is reliable, stable and well repeatable. It provides the useful information for quality evaluation.</p>