Effect of microwave irradiation on expression of heat shock proteins family in primary cultured rat hippocampal neurons / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
; (12): 909-913, 2010.
Article
de Zh
| WPRIM
| ID: wpr-293792
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of microwave irradiation on the expression and regulation of heat shock proteins (HSPs) in primary cultured rat hippocampal neurons.</p><p><b>METHODS</b>Neurons were exposed to 90 mW/cm(2) microwave irradiation for 10 minutes. Western blot was used to determine the expression of HSP27, HSP70, HSP90 and heat shock factor 1 (HSF1) at 0, 3, 6, 12 and 24 hour respectively. Real-time RT-PCR was used to determine the mRNA expression of HSF1. DNA-binding activity of HSF1 was measured by electrophoretic mobility shift assay (EMSA).</p><p><b>RESULTS</b>The protein expression of HSP27 was significantly increased by 22%, 36%, 18% at 3, 6, 12 h, respectively (P < 0.05). The protein expression of HSP70 was significantly increased by 23%, 32%, 26% at 3, 6, 12 h, respectively (P < 0.05, P < 0.01). The protein expression of HSP90 was significantly increased by 27%, 33% at 6, 12 h, respectively (P < 0.05, P < 0.01). The DNA-binding activity of HSF1 was stimulated, however, no significant change of the expression of HSF1 was observed on both the mRNA and protein levels.</p><p><b>CONCLUSION</b>The transcriptional activity of HSF1 is activated by microwave irradiation, which promotes the expression of HSPs. Heat shock response which contributes to establish a cytoprotective state is induced by microwave irradiation in primary cultured rat hippocampal neurons.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Effets des rayonnements
/
Cellules cultivées
/
Protéines du choc thermique
/
Hippocampe
/
Métabolisme
/
Micro-ondes
/
Neurones
Limites du sujet:
Animals
langue:
Zh
Texte intégral:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Année:
2010
Type:
Article