Study on the genotype of Mycobacterium tuberculosis isolates from hospitals in Tianjin / 中华流行病学杂志
Chinese Journal of Epidemiology
;
(12): 785-788, 2007.
Article
Dans Chinois
| WPRIM
| ID: wpr-294236
ABSTRACT
<p><b>OBJECTIVE</b>To explore the characteristics on molecular epidemiology of Mycobacterium tuberculosis isolates from hospitals in Tianjin area.</p><p><b>METHODS</b>One hundred M. tuberculosis isolated strains were collected in succession from August 16th-December 25th, 2005 in Tianjin Haihe Hospital and genotyped by spoligotyping and multiple locus variable number-tandem repeat(VNTR). Data was analyzed by cluster software. Based on the concept of Beijing lineage, it was determinate two sub-groups atypical Beijing strains and W strain/typical family strains by multiplex and real-time PCR. The associations of subgroups with drug resistance and age were assessed by the chi2 test.</p><p><b>RESULTS</b>96 M. tuberculosis strains were genotyped in which 91.7% (88/96) strains belonged to Beijing genotype (including 3 Beijing-like strains) by spoligtyping. VNTR typing could differentiate 60 genotypes among the 88 Beijing genotype strains. 93.2% of the Beijing lineage M. tuberculosis strains of this study belonged to W strain/typical Beijing family strains (82/88). No statistically significant differences were observed in the proportions of the two sub-groups in patients of different age, or drug resistance (P > 0.05).</p><p><b>CONCLUSION</b>The M. tuberculosis Beijing genotype strains were dominated on tuberculosis hospital patients of Tianjin area. The discriminatory power of VNTR typing was higher than that of spoligtyping. The two sub-groups of Beijing lineage had been prevalent in Tianjin, however W strain/typical Beijing family strains were of preponderance.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Tuberculose
/
ADN bactérien
/
Analyse de regroupements
/
Chine
/
Épidémiologie
/
Techniques de typage bactérien
/
Classification
/
Analyse de séquence d'ADN
/
Épidémiologie moléculaire
/
Répétitions minisatellites
Limites du sujet:
Humains
Pays comme sujet:
Asie
langue:
Chinois
Texte intégral:
Chinese Journal of Epidemiology
Année:
2007
Type:
Article
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