Molecular subtyping of Vibrio cholerae isolates by pulsed-field gel electrophoresis in Shenzhen from 1993 / 中华流行病学杂志
Chinese Journal of Epidemiology
;
(12): 491-494, 2007.
Article
Dans Chinois
| WPRIM
| ID: wpr-294307
ABSTRACT
<p><b>OBJECTIVE</b>To determine the genetic relationships between different Vibrio cholerae isolates in Shenzhen from 1993 to 2002.</p><p><b>METHODS</b>Chromosomal DNA from 60 isolates was digested in seakem gold agrose with restriction enzyme Not I and plugs were then analyzed by pulsed-field gel electrophoresis. Pulsed-field gel electrophoresis (PFGE) patterns of V. cholerae isolates were clustered using BioNumerics software.</p><p><b>RESULTS</b>39 distinctive PFGE patterns were identified with each pattern having 20 to 30 bands. Most PFGE patterns were divided into cluster A or cluster B.</p><p><b>CONCLUSION</b>The closely related pandemic clone clusters of V. cholerae strains did exist in Shenzhen. PFGE of V. cholerae could be used for active surveillance and tracking for cholerae.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Phylogenèse
/
Vibrio cholerae
/
Chine
/
Choléra
/
Épidémiologie
/
Classification
/
Électrophorèse en champ pulsé
/
Génétique
/
Méthodes
/
Microbiologie
Limites du sujet:
Humains
Pays comme sujet:
Asie
langue:
Chinois
Texte intégral:
Chinese Journal of Epidemiology
Année:
2007
Type:
Article
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