Study on the application and evaluation of methods for gene and antigen detection in plague surveillance program / 中华流行病学杂志
Chinese Journal of Epidemiology
; (12): 426-429, 2007.
Article
Dans Zh
| WPRIM
| ID: wpr-294323
Responsable en Bibliothèque :
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To apply and evaluate new methods regarding specific gene and antigen detection in plague surveillance program.</p><p><b>METHODS</b>1798 samples from natural foci of plague were tested, using internal quality control multiple-polymerase chain reaction, F1 antigen marked by immuno chromatographic assay and enzyme linked immunosorbent assay. Culture of Yersinia pestis and reverse indirect hemagglutination assay were used as reference diagnostic methods.</p><p><b>RESULTS</b>The overall positive rate of culture on Yersinia pestis together with gene and antigen detection was 7.34%, showing an 16.81% increase when comparing to 6.28% using Yersinia pestis culture method alone. The rate of coincidence was 97.13%.</p><p><b>CONCLUSION</b>The new standard being used for specific gene and antigen detection could increase the positive rate of diagnosis on plague.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Peste
/
Protéines bactériennes
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Virulence
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Yersinia pestis
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Test ELISA
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Réaction de polymérisation en chaîne
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Allergie et immunologie
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Génétique
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Microbiologie
Type d'étude:
Diagnostic_studies
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Evaluation_studies
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Screening_studies
Limites du sujet:
Animals
langue:
Zh
Texte intégral:
Chinese Journal of Epidemiology
Année:
2007
Type:
Article