Study on the application and evaluation of methods for gene and antigen detection in plague surveillance program / 中华流行病学杂志
Chinese Journal of Epidemiology
;
(12): 426-429, 2007.
Article
Dans Chinois
| WPRIM
| ID: wpr-294323
ABSTRACT
<p><b>OBJECTIVE</b>To apply and evaluate new methods regarding specific gene and antigen detection in plague surveillance program.</p><p><b>METHODS</b>1798 samples from natural foci of plague were tested, using internal quality control multiple-polymerase chain reaction, F1 antigen marked by immuno chromatographic assay and enzyme linked immunosorbent assay. Culture of Yersinia pestis and reverse indirect hemagglutination assay were used as reference diagnostic methods.</p><p><b>RESULTS</b>The overall positive rate of culture on Yersinia pestis together with gene and antigen detection was 7.34%, showing an 16.81% increase when comparing to 6.28% using Yersinia pestis culture method alone. The rate of coincidence was 97.13%.</p><p><b>CONCLUSION</b>The new standard being used for specific gene and antigen detection could increase the positive rate of diagnosis on plague.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Peste
/
Protéines bactériennes
/
Virulence
/
Yersinia pestis
/
Test ELISA
/
Réaction de polymérisation en chaîne
/
Allergie et immunologie
/
Génétique
/
Microbiologie
Type d'étude:
Etude diagnostique
/
Études d'évaluation
/
Étude de dépistage
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Chinese Journal of Epidemiology
Année:
2007
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS