Identification of differentially expressed genes in myocardium of patients with heart failure by human whole genomic oligonucleotide microarray-assisted pathways analysis / 中华心血管病杂志
Chinese Journal of Cardiology
;
(12): 120-125, 2009.
Article
Dans Chinois
| WPRIM
| ID: wpr-294766
ABSTRACT
<p><b>OBJECTIVE</b>To identify the differentially expressed gene profiles in myocardium of patients with heart failure using human whole genomic oligonucleotide microarray-assisted pathway analysis.</p><p><b>METHODS</b>Phalanx whole genomic oligonucleotide microarrays were used to detect the gene expression profiles of myocardium in four patients died of heart failure and 4 brain died patients without heart diseases. The microarray findings were confirmed by real-time quantitative reverse transcriptase-polymerase chain reaction. The genes with a threshold of 1.2 times fold-change were selected and BioCarta Pathway and KEGG (Kyoto Encyclopaedia of Genes and Genomes) pathway databases were used to identify functionally related gene pathways.</p><p><b>RESULTS</b>A total of 2806 genes with differentially expression were detected between the failing and non-failing heart samples, expression changes of 399 genes were more than 2-folds. Eleven pathways were identified by BioCarta pathway database and sixteen pathways were identified by KEGG PATHWAY Database.</p><p><b>CONCLUSION</b>Genomic microarray-assisted pathway analysis could help to identify gene expression profiles in failing heart.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
ARN
/
Transduction du signal
/
Génome humain
/
Séquençage par oligonucléotides en batterie
/
Analyse de profil d'expression de gènes
/
Étude d'association pangénomique
/
Gènes
/
Génétique
/
Génotype
/
Défaillance cardiaque
Type d'étude:
Etude diagnostique
/
Étude pronostique
Limites du sujet:
Adulte
/
Adulte très âgé
/
Femelle
/
Humains
/
Mâle
langue:
Chinois
Texte intégral:
Chinese Journal of Cardiology
Année:
2009
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS