Determination in vitro of rat plasma protein binding rate of sinomenine by using microdialysis method / 药学学报

ABSTRACT

<p><b>AIM</b>To determine in vitro the rat plasma protein binding rate by using microdialysis method.</p><p><b>METHODS</b>The binding rate was determined by using microdialysis probe as sampling tools and zero-net flux method as calibrating method. The regression equation was made by the difference of concentrations between the dialysis sample and the perfusate. The x-intercept of regression equation was the free drug concentration (Cf). The plasma protein binding rate was calculated by using the following equation: f = ( C0 - Cf)/C0.</p><p><b>RESULT</b>The binding rate was kept relatively stable in the studied concentration range.</p><p><b>CONCLUSION</b>It is feasible that the plasma protein binding rate can be determined by using microdialysis method.</p>