Transcriptional regulation of cytochrome P450 3A4 by four kinds of traditional Chinese medicines / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 1014-1089, 2008.
Article
Dans Chinois
| WPRIM
| ID: wpr-295420
ABSTRACT
<p><b>OBJECTIVE</b>To screen a group of traditional Chinese medicines with effect on pregnane X receptor (PXR)-mediated transcription regulation of P450 3A4 (CYP3A4); and to study whether they can induce the expression of CYP3A4 with a dose, time-dependent manner.</p><p><b>METHOD</b>Transient cotransfection reporter gene assays were performed with pCI-hPXR-neo, pGL3-CYP3A4-Luc and beta-galactosidase expression plasmid in HepG2 cells.</p><p><b>RESULT</b>Rhizoma Curcumae, Atractylodes lancea, A. macrocaphala and Poria cocos could induce transcriptional expression of CYP3A4. In the dose-effect study, 24 h after induction, 500 mg x L(-1) Rhizoma Curcumae, A. lancea, A. macrocaphala and Poria cocos, respectively, could induce the CYP3A4 gene expression with (6.82 +/- 0.09), (6.76 +/- 0.20), (5.49 +/- 0.13) and (4.97 +/- 0.07) folds, as compared with 0.1% DMSO treated cells. In the time-effect study, 500 mg x L(-1) Rhizoma curcumae, A. lancea, A. macrocaphala and Poria cocos for 48 h could induce the CYP3A4 gene expression with (7.74 +/- 0.54), (7.34 +/- 0.10), (5.54 +/- 0.11) and (5.32 +/- 0.18) folds, compared with 0.1% DMSO treated cells.</p><p><b>CONCLUSION</b>Rhizoma Curcumae, A. lancea, A. macrocaphala and Poria cocos could induce the expression of CYP3A4 gene transcription through activating PXR.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pharmacologie
/
Transcription génétique
/
Médicaments issus de plantes chinoises
/
Récepteurs aux stéroïdes
/
Lignée cellulaire tumorale
/
Cytochrome P-450 CYP3A
/
Génétique
/
Métabolisme
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
China Journal of Chinese Materia Medica
Année:
2008
Type:
Article
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