Biotransformation of podophyllotoxin by cell suspension culture and root culture of Rheum palmatum / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 989-991, 2008.
Article
Dans Chinois
| WPRIM
| ID: wpr-295428
ABSTRACT
<p><b>OBJECTIVE</b>To study the biotransformation of podophyllotoxin by the cell suspension culture and root culture systems of Rheum palmatum.</p><p><b>METHOD</b>Using plant tissue culture technology and HPLC techniques to isolate products. The structures were elucidated by spectroscopic means.</p><p><b>RESULT</b>Cell suspension culture of R. palmatum could convert podophyllotoxin to produce picropodophyllotoxin with the yield of 73.8%, while root culture of R. palmatum could convert podophyllotoxin to produce epipodophyllotoxin and apopodophyllotoxin.</p><p><b>CONCLUSION</b>Podophyllotoxin did not affect the pH value of the media used in tissue cultures. Both cell suspension culture and root culture of R. palmatum can convert podophyllotoxin.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Podophyllotoxine
/
Rheum
/
Structure moléculaire
/
Chimie
/
Chromatographie en phase liquide à haute performance
/
Racines de plante
/
Techniques de culture de tissus
/
Métabolisme
/
Méthodes
langue:
Chinois
Texte intégral:
China Journal of Chinese Materia Medica
Année:
2008
Type:
Article
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