Studies on survival and outgrowth of processes of cultured rat hippocampus neurons in containing selenium and free serum medium / 中华预防医学杂志
Chinese Journal of Preventive Medicine
;
(12): 167-171, 2002.
Article
Dans Anglais
| WPRIM
| ID: wpr-295779
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of selenium on cultured newborn rat's hippocampus neurons survival and outgrowth development.</p><p><b>METHODS</b>Using the technique of primary culture of hippocampal neurons of newborn rat. The different dose of Se (62.5 microgram/L, 125.0 microgram/L, 182.5 microgram/L) were added into the medium at same time. We not only investigated the number of survival of neurons on 1 - 14 d and 1d to 10 d in with and without serum containing-Se medium, but also observed the length outgrowth of the neurite at 16 h, 24 h, 36 h, 48 h during culture.</p><p><b>RESULTS</b>Selenium could obviously enhance the outgrowth of early processed in 10% fetal serum medium and average length of neurite outgrowth is 15 - 20 micrometer more longer than control groups (P < 0.01) and selenium could also increase the livability of neurons and prolong survival time of cultured neurons in serum-free medium.</p><p><b>CONCLUSIONS</b>Selenium may play a very important role for early processed growth and development of hippocampal neurons.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pharmacologie
/
Sélénium
/
Division cellulaire
/
Survie cellulaire
/
Cellules cultivées
/
Neurites
/
Milieux de culture sans sérum
/
Rat Sprague-Dawley
/
Biologie cellulaire
/
Hippocampe
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Chinese Journal of Preventive Medicine
Année:
2002
Type:
Article
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