The expression and role of the transcription factor C/EBPα in chronic myeloid leukemia / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 947-950, 2015.
Article
Dans Chinois
| WPRIM
| ID: wpr-296113
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression and the possible mechanism of the transcription factor C/EBPα in chronic myeloid leukemia(CML).</p><p><b>METHODS</b>Bone marrow samples from 50 CML patients(including 33 patients in chronic phase, 7 in accelerated phase and 10 in blast crisis)and peripheral blood specimens of 20 healthy donors were collected. The expression of C/EBPα gene and the effect of Imatinib on its expression was detected by RT- PCR. C/EBPα gene was inserted into lentivirus expression vector pLVX- EGFP- 3FLAG- Puro by recombinant DNA technology to construct C/EBPα stable expression in K562 cells. Cell proliferation was assayed by CCK-8. The expressions of Foxo3a and Bim genes were detected by RT-PCR.</p><p><b>RESULTS</b>The level of C/EBPα expression was significantly declined in CML patients compared with that of normal control group(P<0.01)and had negative correlation with bcr- abl expression(Spearman r=- 0.505, P<0.01). The stable K562- C/EBPα cell line was successfully established and confirmed by RT-PCR and Western blot. Cell proliferation ability was lower in the K562- C/EBPα group than that in the non- transfection and mock-vehicle groups. The expressions of Foxo3a and Bim genes were 1.06 ± 0.06 and 0.53 ± 0.07, respectively, which was higher than that of nontransfection and mock-vehicle groups(P<0.01, P<0.05).</p><p><b>CONCLUSION</b>C/EBPα expression was decreased in CML patients, overexpression of C/EBPα could inhibit K562 cell growth.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Moelle osseuse
/
Transfection
/
Leucémie myéloïde chronique BCR-ABL positive
/
Crise blastique
/
Études cas-témoins
/
Cycle cellulaire
/
Cellules K562
/
Protéine alpha liant les séquences stimulatrices de type CCAAT
/
Prolifération cellulaire
/
Mésilate d'imatinib
Type d'étude:
Étude observationnelle
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Hematology
Année:
2015
Type:
Article
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