Expression of Chimeric Influenza Hemagglutinin Antigen (cH7/3) using the Baculovirus Expression System and Identification of its Biological Activities / 病毒学报
Chinese Journal of Virology
;
(6): 524-529, 2015.
Article
Dans Chinois
| WPRIM
| ID: wpr-296253
ABSTRACT
Hemagglutinin (HA) contains a head domain with a high degree of variability and a relatively conserved stem region. HA is the major viral antigen on the surface of the influenza virus. To define the biologic activities of chimeric HA bearing different head domains and stem regions or their potential use, a HA chimeric gene containing the head domain of the H7 subtype virus and stem region of the H3 subtype virus was modified and expressed using a baculovirus expression vector. Then, the secreted protein was purified and its biologic activities characterized. Approximately 1.4 mg/mL cH7/3 HA could be obtained, and its molecular weight was ≈ 70 kD. The trimer form of cH7/3 protein had hemagglutination activity and could be recognized by specific antibodies. The method described here can be used for further studies on the screening of HA stem-reactive antibodies or the development of vaccines with conserved epitopes.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Virologie
/
Vaccins antigrippaux
/
Expression des gènes
/
Baculoviridae
/
Glycoprotéine hémagglutinine du virus influenza
/
Allergie et immunologie
/
Grippe humaine
/
Vecteurs génétiques
/
Génétique
/
Hémagglutination
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Virology
Année:
2015
Type:
Article
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