Long Noncoding RNA Expression Signatures of Abdominal Aortic Aneurysm Revealed by Microarray / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 713-723, 2016.
Article
Dans Anglais
| WPRIM
| ID: wpr-296548
ABSTRACT
<p><b>OBJECTIVE</b>This study is aimed at observing the role of long noncoding RNAs (lncRNAs) in the pathogenesis of abdominal aortic aneurysm (AAA).</p><p><b>METHODS</b>LncRNA and mRNA expression signatures of AAA tissues and normal abdominal aortic tissues (NT) were analyzed by microarray and further verified by Real-time quantitative reverse-transcription PCR (qRT-PCR). The lncRNAs-mRNAs targeting relationships were identified using computational analysis. The effect of lnc-ARG on 5-lipoxygenase (ALOX5) expression was tested in HeLa cells.</p><p><b>RESULTS</b>Differential expressions of 3,688 lncRNAs and 3,007 mRNAs were identified between AAA and NT tissues. Moreover, 1,284 differentially expressed long intergenic noncoding RNAs and 206 differentially expressed enhancer-like lncRNAs adjacent to protein-coding genes were discerned by bioinformatics analysis. Some differentially expressed lncRNAs and mRNAs between AAA and normal tissue samples were further verified using qRT-PCR. A co-expression network of coding and noncoding genes was constructed based on the correlation analysis between the differentially expressed lncRNAs and mRNAs. In addition, the lnc-ARG located within the upstream of ALOX5 was sorted as a noncoding transcript by analyzing the protein-coding potential using computational analysis. Furthermore, we found that lnc-ARG can decrease the mRNA level of ALOX5 and reactive oxygen species production in HeLa cells.</p><p><b>CONCLUSION</b>This study revealed new lncRNA candidates are related to the pathogenesis of AAA.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anévrysme de l'aorte abdominale
/
Séquençage par oligonucléotides en batterie
/
Analyse de profil d'expression de gènes
/
ARN long non codant
/
Génétique
/
Métabolisme
Limites du sujet:
Adulte très âgé
/
Femelle
/
Humains
/
Mâle
langue:
Anglais
Texte intégral:
Biomedical and Environmental Sciences
Année:
2016
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS