Effect of Smo SiRNA-mediated Hedgehog Signaling Pathway Inhibition on Palatal Fusion / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 594-598, 2016.
Article
Dans Anglais
| WPRIM
| ID: wpr-296563
ABSTRACT
We used Smo siRNA to inhibit hedgehog signaling pathway in embryonic day (E) 13 palatal shelves in organ culture. SiRNA 4 was chosen as the most efficient from four synthesized Smo siRNAs. Palatal shelf fusion rate of 4 μg/mL cyclopamine group was the lowest and significantly lower than that of blank control group (P<0.05), and that of siRNA 4 group was also lower than that of blank control group (P=0.183). At 48 h after transfection, Smo protein level of siRNA 4 group was 64.8% lower than that of blank control group (P<0.05), and Gli1 protein level of 4 μg/mL cyclopamine group was 68.9% lower than that of blank control group (P<0.05). Hedgehog signaling pathway inhibition decreased palatal fusion in organ culture, probably owing to downregulation of Smo and Gli1 proteins.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Palais
/
Transduction du signal
/
Embryologie
/
Petit ARN interférent
/
Facteurs de transcription Krüppel-like
/
Protéines Hedgehog
/
Protéine à doigts de zinc Gli3
/
Protéine à doigts de zinc Gli2
/
Génétique
/
Métabolisme
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Biomedical and Environmental Sciences
Année:
2016
Type:
Article
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