Exploring Genome-wide DNA Methylation Profiles Altered in Kashin-Beck Disease Using Infinium Human Methylation 450 Bead Chips / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 539-543, 2016.
Article
Dans Anglais
| WPRIM
| ID: wpr-296570
ABSTRACT
To understand how differentially methylated genes (DMGs) might affect the pathogenesis of Kashin-Beck disease (KBD). Genome-wide methylation profiling of whole blood from 12 matched KBD and controls pairs was performed using a high-resolution Infinium 450 K methylation array. In total, 97 CpG sites were differentially methylated in KBD compared to the normal controls; of these sites, 36 sites were significantly hypermethylated (covering 22 genes) and 61 sites were significantly hypomethylated (covering 34 genes). Of these genes, 14 significant pathways were identified, the most significant P value pathway was type I diabetes mellitus pathway and pathways associated with autoimmune diseases and inflammatory diseases were included in this study. Subsequently, 4 CpG sites in HLA-DRB1 were validated using bisulfite sequencing polymerase chain reaction (BSP) in articular cartilage, and the results showed significant differences in the methylation status between KBD and controls, consistent with the results of the high-resolution array. These results suggested that differences in genome-wide DNA methylation exist between KBD and the controls, and the biological pathways support the autoimmune disease and inflammatory disease hypothesis of KBD.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Variation génétique
/
Études cas-témoins
/
Analyse de regroupements
/
Ilots CpG
/
Méthylation de l'ADN
/
Séquençage par oligonucléotides en batterie
/
Étude d'association pangénomique
/
Maladie de Kashin-Beck
/
Génétique
Type d'étude:
Étude observationnelle
/
Facteurs de risque
Limites du sujet:
Adulte
/
Femelle
/
Humains
langue:
Anglais
Texte intégral:
Biomedical and Environmental Sciences
Année:
2016
Type:
Article
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