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NKX2.5 and TBX5 gene mutations in in vitro fertilization children with congenital heart disease / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics ; (12): 652-657, 2017.
Article Dans Chinois | WPRIM | ID: wpr-297232
ABSTRACT
<p><b>OBJECTIVE</b>To explore the differences of NKX2.5 and TBX5 gene mutations between in vitro fertilization (IVF) children with congenital heart disease (CHD) and naturally conceived children with CHD.</p><p><b>METHODS</b>Blood samples from 68 IVF children with CHD and 98 naturally conceived children with CHD were collected. The mutations in coding regions 1 and 2 of the NKX2.5 gene, and coding regions 4, 5, and 8 of the TBX5 gene were examined by polymerase chain reaction (PCR) and DNA sequencing.</p><p><b>RESULTS</b>An A-to-G mutation at nucleotide 63 (c.63A>G) in coding region 1 of the NKX2.5 gene was found in both IVF and naturally conceived children with CHD. There were no significant differences in genotype and allele frequencies at c.63A>G locus of the NKX2.5 gene between the two groups. No mutations were detected in coding region 2 of the NKX2.5 gene and coding regions 4, 5 and 8 of the TBX5 gene.</p><p><b>CONCLUSIONS</b>There is no difference in NKX2.5 and TBX5 gene mutations between IVF and naturally conceived children with CHD. Therefore, it is presumed that assisted reproductive technology may not lead to mutations in the NKX2.5 and TBX5 genes.</p>
Sujets)
Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Fécondation in vitro / Protéines à domaine boîte-T / Génétique / Cardiopathies congénitales / Mutation Limites du sujet: Enfant d'âge préscolaire / Femelle / Humains / Bébé / Mâle / Nouveau-né langue: Chinois Texte intégral: Chinese Journal of Contemporary Pediatrics Année: 2017 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Fécondation in vitro / Protéines à domaine boîte-T / Génétique / Cardiopathies congénitales / Mutation Limites du sujet: Enfant d'âge préscolaire / Femelle / Humains / Bébé / Mâle / Nouveau-né langue: Chinois Texte intégral: Chinese Journal of Contemporary Pediatrics Année: 2017 Type: Article