Effects of triptolide on prostate carcinoma in mouse RM-1 cells / 中华男科学杂志
National Journal of Andrology
;
(12): 237-241, 2007.
Article
Dans Chinois
| WPRIM
| ID: wpr-297746
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of Triptolide (TL) on the growth of prostate carcinoma cell line, and analyze its function and mechanism in anti-prostate cancer.</p><p><b>METHODS</b>MTT experiments were performed to examine the inhibiting effect of TL on the proliferation of RM-1 cells, cell morphological changes observed by acridine staining, cellular cycles and apoptosis peak analyzed by flow cytometry, the apoptosis fracture zone investigated with DNA electrophoresis, and the expressions of caspase-3 and bcl-2 mRNA in RM-1 cells examined by RT-PCR.</p><p><b>RESULTS</b>The results of MTT experiments showed that after the treatment of TL (5, 10, 20, 40 and 80 ng/ml), the RM-1 cell proliferation inhibition rates were 9.8%, 25.1%, 39.2%, 48.8% and 53.2% respectively; 12, 24, 36 and 48 hours after the treatment of TL (10 and 20 ng/ml), the cell proliferation inhibition rates were 8.4%, 25.1%, 36.1%, 42.4% and 10.2%, 39.2%, 50.2% and 58.5% respectively. Acridine staining after the TL treatment revealed nucleus condensation, cell membrane invagination, irregular orange particles in the cells and apoptosis morphological changes; flow cytometry tests showed that 48 hours after the TL treatment (10, 20 ng/ml) of RM-1 cells, an obvious apoptosis peak appeared before the G1 stage; 24, 36 and 48 hours after it, DNA "trapezoid" strips could be seen; the caspase-3 mRNA expression in the TL treated cells was higher, and the bcl-2 mRNA expression was lower than in the controls.</p><p><b>CONCLUSION</b>TL can decrease bcl-2 expression, increase caspase-3 expression, induce apoptosis of prostate carcinoma cells, and consequently inhibit the proliferation of RM-1 cells in mice.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
Pharmacologie
/
Phénanthrènes
/
Tumeurs de la prostate
/
ARN messager
/
Apoptose
/
Antinéoplasiques alcoylants
/
Protéines proto-oncogènes c-bcl-2
/
RT-PCR
/
Lignée cellulaire tumorale
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
National Journal of Andrology
Année:
2007
Type:
Article
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