The development of neutralizing human antibodies against rabies virus / 病毒学报
Chinese Journal of Virology
;
(6): 271-275, 2010.
Article
Dans Chinois
| WPRIM
| ID: wpr-297871
ABSTRACT
A combinatorial human Fab library to the rabies virus was constructed using antibody genes derived from the blood of vaccinated donors. The library were panned and selected on purified rabies virus particles of aG or CTN strain with phage display. Eleven unique human Fab antibodies specific for the rabies virus glycoprotein were obtained by ELISA, IFA and DNA sequences analysis of these antibodies. Among these Fab antibodies, five human Fab antibodies were converted to full-length human IgG antibodies with recombinant baculovirus system. The five full-length human IgG antibodies were tested in vitro for rabies virus neutralization, resulting in all specificities to neutralize the virus. The obtained human anti-rabies antibodies lay the basis for the production of cocktail of anti-rabies monoclonal antibody with chinese intellectual property.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Rage (maladie)
/
Virus de la rage
/
Virologie
/
Immunoglobuline G
/
Fragments Fab d'immunoglobuline
/
Tests de neutralisation
/
Données de séquences moléculaires
/
Lignée cellulaire
/
Séquence d'acides aminés
/
Allergie et immunologie
Limites du sujet:
Animaux
/
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Virology
Année:
2010
Type:
Article
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