Analysis of full-length gene sequence of a rabies vaccine strain CTN-1 for human use in China / 病毒学报
Chinese Journal of Virology
;
(6): 195-201, 2010.
Article
Dans Chinois
| WPRIM
| ID: wpr-297884
ABSTRACT
CTN-1 is one of the rabies vaccine strains for human use in China, but there has been no report on the full-length gene sequence of CTN-1. In this study, the full-length gene of CTN-1 was amplified by RT-PCR, each PCR product was cloned into T vector and then sequenced, assemblied and compared with other vaccine strains as well as the wild Chinese rabies isolates. The phylogenetic tree of G gene was constructed and the genetic homology was analyzed. The results revealed that CTN-1 was 11 925nt (GenBank accession number FJ959397)in length and belonged to the genotype I. The full-length nucleotide homologies among CTN-1 and other rabies virus strains were between 81.5%-93.4%, of which the lowest 81.5% was between CTN-1 strain and bat isolate SHBRV, and the highest 93.4% was between CTN-1 and Chinese isolate HN10. The phylogenetic analysis revealed that the majority of Chinese isolates could be grouped into the same clade with the CTN-1 strain, but aG and some vaccine strains from abroad such as Flury, PM, PV, ERA, RC-HL and a few Chinese strains were grouped in another clade. Comparsion of the G protein genes also showed that the homologies among CTN-1 and most of the Chinese isolates were higher than that of the other vaccine strains to those Chinese strains. Therefore, it suggests that the CTN-1 strain is more suitable and rational to be used for the production of rabies inactivated vaccine in China than the others.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Phylogenèse
/
Rage (maladie)
/
Virus de la rage
/
Virologie
/
Données de séquences moléculaires
/
Vaccins antiviraux
/
Similitude de séquences d'acides nucléiques
/
Alignement de séquences
/
Classification
/
Génome viral
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Virology
Année:
2010
Type:
Article
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