Preparation and identification of dengue virus with deletion mutation of capsid protein / 南方医科大学学报
Journal of Southern Medical University
; (12): 31-37, 2007.
Article
de Zh
| WPRIM
| ID: wpr-298251
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To generate rescued viruses with deletion mutation of capsid protein from dengue virus type 2 isolated in China (DEN2-43).</p><p><b>METHODS</b>On the basis of infectious full-length cDNA clone pD212 of DEN2-43 strain virus, the deletion mutants were constructed by fusion PCR, from which the rescued viruses with deletion mutation of capsid protein were generated by transcription in vitro and electroporation.</p><p><b>RESULT AND CONCLUSION</b>Sequence analysis demonstrated that the deletion mutations had been successfully inserted into the rescued viruses obtained. These mutant viruses may hold the key for elucidating the effects of deletion mutation of capsid protein on the biological characteristics of dengue virus.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Transcription génétique
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Réplication virale
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Analyse de mutations d'ADN
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Données de séquences moléculaires
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Séquence nucléotidique
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Similitude de séquences d'acides nucléiques
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Lignée cellulaire
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Séquence d'acides aminés
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Clonage moléculaire
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Délétion de séquence
Type d'étude:
Prognostic_studies
Limites du sujet:
Animals
langue:
Zh
Texte intégral:
Journal of Southern Medical University
Année:
2007
Type:
Article