Viral surveillance on rabies in Hunan province,in 2006 / 中华流行病学杂志

ABSTRACT

Objective To understand the source and distribution of rabies virus(RV)in Hunan province with viral surveillance in order to provide scientific measures for prevention and control on rabies.Methods Brain samples from healthy-looking domestic dogs were collected in the agricultural markets at the dist6cLs of high.middle,and lOW incidence rates and detected by direct Immunofluorescence assay (DFA).Positive samples would be further detected by RT-PCR and the surveillance samples were detected bv RT.PCR.The positive samples detected bv RT-PCR were sequenced with N gene.Results The infection rate of thosc healthy-looking domestic dogs with rabies virus was 2.78％in Hunan province in 2005.23 positive samples’N gene were sequenced and their similarities were 88.8％-100.0％.The results indicated that Hunan rabies virus N gene aberrance was mainly synonymous aberrance and did not CKITy obvious regional characteristics.The rabies virus were circulating among different districts in Hunan province,and the neighboring provinces such as Guizhou,Hubei，GuangxiComparison of immunity to measles between floating and local population,Jiangsu and Henan.There were no positive samples detected in salivary，blood and urine samples.There was one positive sample detected in two skin samples.Conclusion There are dogs infected with rabies virus found in Hunan province and this study showed that rabies virus detected in Hunan had a close genetic relationship with those rabies idcntified in other provinces,suggesting that study on the immunity and management of dog related rabies should be strengthened.