Determination of oleuropein in stem of Syringa oblata from different districts by HPLC / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 304-306, 2009.
Article
Dans Chinois
| WPRIM
| ID: wpr-298410
ABSTRACT
<p><b>OBJECTIVE</b>To develop an HPLC method for the determination of oleuropein in Syringa oblata.</p><p><b>METHOD</b>An Aigilent ZORBAX SB- C18 column (4.6 mm x 250 mm, 5 microm) was used. The mobile phase was acetonitrile-water (21 79). The flow rate was 1.0 mL x min(-1). The detection wavelenghth was set at 232 nm and the column temperature was 30 degrees C.</p><p><b>RESULT</b>The linear range of oleuropein were from 0.011 62 g x L(-1) to 1.162 g x L(-1). The average recovery was 98.7% with RSD 2.5% (n=9).</p><p><b>CONCLUSION</b>The method is reliable, accurate and specific. It can be used for quality control of the stem of Syringa oblata.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Acétonitriles
/
Pyrannes
/
Vasodilatateurs
/
Benzoates
/
Médicaments issus de plantes chinoises
/
Chimie
/
Chromatographie en phase liquide à haute performance
/
Syringa
/
Iridoïdes
/
Méthodes
langue:
Chinois
Texte intégral:
China Journal of Chinese Materia Medica
Année:
2009
Type:
Article
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