Protective effects of recombinant SCR15-18 domain of human soluble complement receptor type 1 on myocardial ischemia and reperfusion injury / 中华心血管病杂志
Chinese Journal of Cardiology
; (12): 1037-1040, 2007.
Article
de Zh
| WPRIM
| ID: wpr-299533
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the protective effects of recombinant SCR15-18 domain of human soluble complement receptor type 1 (sCR1-SCR-15-18) in rats underwent myocardial ischemia and reperfusion (I/R).</p><p><b>METHODS</b>Sprague-Dawley rats were randomly divided into three groups (n = 12 each group): sham (SO); 30 min ischemia/3h reperfusion (I/R) and I/R plus sCR1-SCR15-18 (15 mg/kg before I/R, sCR1). Serum LDH, CK and cardiac myeloperoxidase (MPO) activity were measured. Infarct size, myocardial histopathological changes and myocardial C3c were also compared among groups.</p><p><b>RESULTS</b>Infarct size [(16.1 +/- 3.3)% vs. (22.9 +/- 3.0)%, infarct zone/left ventricular mass, P < 0. 05] and CK [(2532.5 +/- 597.1) U/L vs. (3400.9 +/- 534.9) U/L, P < 0. 05] and LDH [(5436.2 +/- 611.3) U/L vs. (6572.0 +/- 476.3) U/L, P < 0. 05] as well as MPO activity in infarct zone [(0.81 +/- 0.14) U/g vs. (1.12 +/- 0.13) U/g, P < 0.05] were significantly decreased post sCR1 compared to I/R group. sCR1 also significantly attenuated histological myocardial injury and reduced the deposition of C3c in infarct zone.</p><p><b>CONCLUSION</b>sCR1-SCR15-18 protein exerts cardioprotective effects in this rat I/R model.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Anatomopathologie
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Protéines recombinantes
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Récepteurs au complément
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Lésion de reperfusion myocardique
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Récepteurs au C3b du complément
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Rat Sprague-Dawley
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Myeloperoxidase
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Creatine kinase
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Utilisations thérapeutiques
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Modèles animaux de maladie humaine
Limites du sujet:
Animals
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Humans
langue:
Zh
Texte intégral:
Chinese Journal of Cardiology
Année:
2007
Type:
Article