Determination of polysaccharides content of Gentiana farreri from different producing areas based on anthrone-sulfuric acid method / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2774-2776, 2014.
Article
Dans Chinois
| WPRIM
| ID: wpr-299856
ABSTRACT
Study a method for the detemination of the content of polysaccharides in Gentiana farreri, and analysis of the content of polysaccharides from different producing areas. The results showed that using the anthrone-sulfuric acid method, simple operation, accurate result. Sample was measured at 620 nm absorbance after anthrone-sulfuric acid color, at this wavelength, solution absorption and glucose showed a good linear relationship; The linearity was in the range of 0.01-0.07 g x L(-1) (r = 0.996 7). The recovery rate was 99.41%, with RSD of 2.0%. Considering the experimental conditions, to determine the solid-liquid ratio 160, extracting time 50 min, concentration of ethanol 80%. The mass fraction of polysaccharides was the highest to reached 0.743% in G. farreri from Gansu Xiahe. This experiment has laid a good foundation for further study on G. farreri.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Polyosides
/
Acides sulfuriques
/
Facteurs temps
/
Modèles linéaires
/
Chimie
/
Techniques de chimie analytique
/
Reproductibilité des résultats
/
Gentiana
/
Géographie
/
Anthracènes
Type d'étude:
Étude pronostique
langue:
Chinois
Texte intégral:
China Journal of Chinese Materia Medica
Année:
2014
Type:
Article
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