Effect of ischemic preconditioning on cytokines during lung ischemia-reperfusion injury / 中华外科杂志
Chinese Journal of Surgery
; (12): 545-547, 2003.
Article
Dans Zh
| WPRIM
| ID: wpr-299990
Responsable en Bibliothèque :
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the protective role of ischemic preconditioning (IPC) during lung ischemia-reperfusion (I/R) injury and its influence on inflammatory cytokine production.</p><p><b>METHODS</b>In vivo I/R injury of rabbit was induced by blocking hilum of the left lung. The wet/dry ratio of the lung, lung permeability index and neutrophils percentage in bronchoalveolar lavage fluid (BALF) were detected as indexes of the lung injury. Serum levels of tumor necrosis factor alpha (TNFalpha), interleukin-6 (IL-6) and interleukin-8 (IL-8) were also detected using enzyme-linked immunosorbent assay. The protective role of IPC and its influence on inflammatory cytokine production were observed.</p><p><b>RESULTS</b>The wet/dry ratio of the lung, lung permeability index and neutrophils percentage in BALF of I/R group were 9.73 +/- 1.14, (41.62 +/- 5.77) x 10(-4) and (58.1 +/- 10.0)% respectively. The IPC group indexes were 6.23 +/- 0.69, (20.31 +/- 4.03) x 10(-4) and (23.8 +/- 5.2)% respectively. There was a significant difference between the two groups (P < 0.01). Serum levels of TNFalpha, IL-6 and IL-8 of I/R group were (0.9078 +/- 0.1062), (0.2137 +/- 0.0598) and (0.7211 +/- 0.0979) ng/ml respectively. The IPC group indexes were (0.7478 +/- 0.0843), (0.1271 +/- 0.0089) and (0.5903 +/- 0.0746) ng/ml respectively, significantly lower than that of I/R group (P < 0.01).</p><p><b>CONCLUSIONS</b>Lung IPC has a marked protection effect against I/R injury. The effect was related to its inhibition of inflammatory cytokines such as TNFalpha, IL-6 and IL-8, thus reducing activation and infiltration of neutrophils.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Sang
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Lésion d'ischémie-reperfusion
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Répartition aléatoire
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Cytokines
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Interleukine-8
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Interleukine-6
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Facteur de nécrose tumorale alpha
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Préconditionnement ischémique
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Modèles animaux de maladie humaine
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Poumon
Type d'étude:
Prognostic_studies
Limites du sujet:
Animals
langue:
Zh
Texte intégral:
Chinese Journal of Surgery
Année:
2003
Type:
Article