Differential gene expression of liver carcinoma cell after transfection of by ARL-1 with microarray / 中华外科杂志
Chinese Journal of Surgery
;
(12): 180-182, 2003.
Article
Dans Chinois
| WPRIM
| ID: wpr-300055
ABSTRACT
<p><b>OBJECTIVE</b>To study the difference of the gene expression profile and to identify the different expression after transfection of the ARL-1 gene.</p><p><b>METHODS</b>The cDNA probes were synthesized from total RNA of study group and control group, which was differentially hybridized to cDNA chips and confirmed by a gene specific semiquantitative reverse transcription polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>Six kinds of gene expression were increased and 9 kinds of gene expression were decreased. The findings were correlated with protein metabolism, signal pathway, metastasis, and drug resistance.</p><p><b>CONCLUSIONS</b>cDNA chips showed that gene expression profile of liver carcinoma cell was changed after transfection of the ARL-1 gene. It is a useful method in understanding the mechanism of drug resistance.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Transfection
/
Aldose reductase
/
Résistance aux médicaments antinéoplasiques
/
Séquençage par oligonucléotides en batterie
/
Analyse de profil d'expression de gènes
/
Traitement médicamenteux
/
Génétique
/
Tumeurs du foie
Type d'étude:
Étude pronostique
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Surgery
Année:
2003
Type:
Article
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