The effect of ultra high molecular weight polyethylene particles on macrophage / 中华外科杂志
Chinese Journal of Surgery
;
(12): 852-855, 2006.
Article
Dans Chinois
| WPRIM
| ID: wpr-300597
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of ultra high molecular weight polyethylene (UHMWPE) particles on macrophages and evaluate the expression of NFAT2, a key transcriptional factor for osteoclast differentiation.</p><p><b>METHODS</b>From November 2004 to February 2005, macrophages were co-cultured with UHMWPE particles. When observed at different times, the proliferation activity of macrophages was analyzed by MTT and the expression of calcineurin (CaN) and NFAT2 by immunohistochemical and RT-PCR method respectively.</p><p><b>RESULTS</b>The macrophages phagocytosed UHMWPE particles in an early time, the expression of CaN and NFAT2 was increased, while the proliferation activity was not enhanced.</p><p><b>CONCLUSIONS</b>UHMWPE particles can stimulate macrophages to phagocytose significantly, and enhance the expression of the transcriptional factor NFAT2.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Phagocytose
/
Pharmacologie
/
Physiologie
/
Polyéthylènes
/
Techniques in vitro
/
ARN messager
/
Facteurs de transcription NFATC
/
Génétique
/
Prothèse articulaire
/
Macrophages
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Chinese Journal of Surgery
Année:
2006
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS