Effects of 60 Hz electromagnetic field exposure on testicular germ cell apoptosis in mice / 亚洲男科学杂志(英文版)
Asian Journal of Andrology
;
(6): 29-34, 2004.
Article
Dans Anglais
| WPRIM
| ID: wpr-300869
ABSTRACT
<p><b>AIM</b>To evaluate the effects of 60 Hz extremely low frequency (ELF) elelctromagnetic field (EMF) exposure on germ cell apoptosis in the testis of mice.</p><p><b>METHODS</b>Adult male BALB/c mice (7 weeks of age) were exposed to a 60 Hz EMF of 0.1 mT or 0.5 mT for 24 h/day. A sham-exposed group served as the control. After 8 weeks of exposure, the mice were sacrificed. Germ cell apoptosis in the testis was assessed by histopathological examination, the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling assay (TUNEL) and flow cytometric examination of isolated spermatogenic cells stained with 7 aminoactinomycin D (7-AAD).</p><p><b>RESULTS</b>EMF exposure did not significantly affect the body and testis weights, but significantly increased the incidence of germ cell death. The distinguishing morphological feature of EMF exposure was a decrement in the number of well organized seminiferous tubules. Quantitative analysis of TUNEL-positive germ cells showed a significantly higher apoptotic rate in the 0.5 mT exposed mice than that in the sham controls (P<0.05), while the difference between the two exposed groups was insignificant. The TUNEL-positive cells were mainly spermatogonia. In flow cytometry analysis, the percentage of live cells [forward scatter count (FSC)(high)7-AAD(-)] was lower in the exposed groups than that in the controls (Figure 5A), but the decrease in viability was not statistically significant.</p><p><b>CONCLUSION</b>Continuous exposure to ELF EMF may induce testicular germ cell apoptosis in mice.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Taille d'organe
/
Spermatozoïdes
/
Testicule
/
Poids
/
Apoptose
/
Méthode TUNEL
/
Biologie cellulaire
/
Champs électromagnétiques
/
Cytométrie en flux
/
Souris de lignée BALB C
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Asian Journal of Andrology
Année:
2004
Type:
Article
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