Effect of crocetin on cardiac hypertrophy induced by overloading pressure in rats / 药学学报

ABSTRACT

<p><b>AIM</b>To study the influence of crocetin on cardiac hypertrophy induced by overloading pressure in rats.</p><p><b>METHODS</b>The model of cardiac hypertrophy was produced by overloading pressure in rats. The animals were divided into five groups sham-operation group (0.5% CMC-Na, ig), model group (operation + 0.5% CMC-Na, ig), captopril group (operation + 50 mg x kg(-1), ig), crocetin I (100 mg x kg(-1), ig) and crocetin II (50 mg x kg(-1), ig). All animals were treated for 30 d by ig. Then, cardiac indexes were examined. The activity of ATPase and the hydroxyproline content in heart were assayed by colorimetric analysis. Matrix metalloproteinases (MMPs) activity was assayed by SDS-PAGE zymography.</p><p><b>RESULTS</b>Compared with the model group, crocetin was found to significantly reduce the cardiac indexes and the content of hydroxyproline in heart, increase the activity of Na+ , K+ -ATPase, Ca2+, Mg2+ -ATPase and inhibit MMPs activity.</p><p><b>CONCLUSION</b>The activity of MMPs may play a key role in the cardiac hypertrophy induced by overloading pressure, and proprably as a result of decreasing the activity of MMPs. Crocetin was shown to prevent remodeling of cardiac hypertrophy induced by overloading pressure.</p>