The changes of extracellular matrix in adult degenerative nucleus pulposus cells with stiring microcarrier culture system in vitro / 中华外科杂志
Chinese Journal of Surgery
; (12): 432-436, 2013.
Article
Dans Zh
| WPRIM
| ID: wpr-301265
Responsable en Bibliothèque :
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the biological effect on the synthesis of the extracellular matrix (ECM) in the cultivation of adult degenerative nucleus pulposus cells using the stiring microcarrier system in vitro.</p><p><b>METHODS</b>Thirty-four specimens were collected after intervertebral fusion operations of the patients with intervertebral disc herniation diseases from September 2005 to May 2009. The specimens were then randomly allocated into 2 groups for in vitro cultivation: monolayer culture group and microcarrier culture group. On the exponential phase, SP-ABC immunohistochemical staining and Western blot quantitative analysis were conducted in the two groups to detect the collagen type I and II. Proteoglycan contents of two groups in different growth phases were detected with (35)S-sulfate incorporation assay.</p><p><b>RESULT</b>The expressions of collagen type I and II in microcarrier culture group were significantly higher than those in monolayer culture group: SP-ABC immunohistochemical staining (collagen type I: 32.5 ± 4.4 vs. 15.2 ± 1.2, t = 2.871, P < 0.01; collagen type II: 43.6 ± 4.1 vs. 23.1 ± 2.2, t = 2.375, P < 0.05); Western blot quantitative analysis (collagen type I: 0.62 ± 0.08 vs. 0.50 ± 0.06, t = 3.327, P < 0.01; collagen type II: 1.46 ± 0.08 vs. 0.86 ± 0.04, t = 2.453, P < 0.05). Nucleus pulposus cells cultivated in stiring microcarrier system showed significantly increased proteoglycan synthesis than monolayer culture group does on both exponential phase and stationary phase (exponential phase: 34 821 ± 312 vs. 21 046 ± 673, t = 2.134, P < 0.05; stationary phase: 45 134 ± 175 vs. 32 193 ± 713, t = 2.801, P < 0.01).</p><p><b>CONCLUSIONS</b>The expression of collagen type I, II and proteoglycan of adult degenerative nucleus pulposus cells are positive regulated by the stiring microcarrier system, which can be used in the mass amplification of the adult degenerative nucleus pulposus cells.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Protéoglycanes
/
Répartition aléatoire
/
Collagène
/
Techniques de culture cellulaire
/
Biologie cellulaire
/
Matrice extracellulaire
/
Disque intervertébral
/
Métabolisme
Type d'étude:
Clinical_trials
Limites du sujet:
Adult
/
Aged
/
Female
/
Humans
/
Male
langue:
Zh
Texte intégral:
Chinese Journal of Surgery
Année:
2013
Type:
Article