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Differential expression of RPL6/Taxreb107 in drug resistant gastric cancer cell line SGC7901/ADR and its correlation with multiple-drug resistance / 中华肿瘤杂志
Zhonghua zhong liu za zhi ; (12): 21-25, 2003.
Article de Zh | WPRIM | ID: wpr-301915
Bibliothèque responsable: WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the differential expression of RPL6/Taxreb107 between drug-resistant gastric cancer cell line SGC7901/ADR and gastric cancer cell line SGC7901 as well as its correlation with multiple-drug resistance (MDR) in gastric cancer cells.</p><p><b>METHODS</b>Total RNA was extracted from SGC7901 and SGC77901/ADR, with internal control RT-PCR, Northern blot, gene cloning and expression, construction of eukaryotic expression vector, gene transfection by electroporation. The accumulation and retention of ADR in transiently transfected cell was detected by flow cytometry.</p><p><b>RESULTS</b>The internal control RT-PCR and Northern blot showed high RPL6/Taxreb107 expression in SGC7901/ADR cell line. Sense and antisense eukaryonic expression vectors demonstrated by double enzyme digestion were successfully transfected into gastric cancer cell line SGC7901 and SGC7901/ADR respectively by electroporation. The accumulation and retention of ADR detected 48 hours after transfection showed that RPL6 gene had shown effect on drug resistance in gastric cancer cell.</p><p><b>CONCLUSION</b>The high expression of RPL6/Taxreb107 in drug resistant gastric cancer cell shows its correlation with multiple-drug resistance in gastric cancer.</p>
Sujet(s)
Texte intégral: 1 Indice: WPRIM Sujet Principal: Anatomopathologie / Physiologie / Tumeurs de l'estomac / Cellules cancéreuses en culture / Statistiques comme sujet / Multirésistance aux médicaments / Résistance aux médicaments antinéoplasiques / Protéines de liaison à l'ADN / Métabolisme Limites du sujet: Humans langue: Zh Texte intégral: Zhonghua zhong liu za zhi Année: 2003 Type: Article
Texte intégral: 1 Indice: WPRIM Sujet Principal: Anatomopathologie / Physiologie / Tumeurs de l'estomac / Cellules cancéreuses en culture / Statistiques comme sujet / Multirésistance aux médicaments / Résistance aux médicaments antinéoplasiques / Protéines de liaison à l'ADN / Métabolisme Limites du sujet: Humans langue: Zh Texte intégral: Zhonghua zhong liu za zhi Année: 2003 Type: Article