Allelic analysis on chromosome 5 in sporadic colorectal cancer patients / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 458-460, 2002.
Article
Dans Chinois
| WPRIM
| ID: wpr-301988
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate and map the putative tumor suppressor loci on chromosome 5 involved in tumor progress or metastasis.</p><p><b>METHODS</b>Chromosome 5 of 83 patients with sporadic colorectal cancer was systemically screened. Fifteen microsatellite marker primers labeled with 3 different fluorescents were used to amplify the corresponding loci of the genome DNA. The PCR products were electrophoresed on a 377 PRISM sequencer and the fluorescent signals were analyzed with Genotyper and Genescan software.</p><p><b>RESULTS</b>The highest loss of heterozygosity (LOH) ratio was found at D5S416 (48.15%) on 5p and at D5S471 (38.71%) on 5q. The region (5q13.3 - 31), where D5S471 and 3 neighboring loci (D5S428, D5S2027 and D5S2115) reside, presented high frequent LOH.</p><p><b>CONCLUSION</b>The deletion of APC, MCC, CTNNA1 and IL cluster in the 5q 13.3 - 31.1 area play important role in the tumorogenesis of colorectal cancer, and the expected existence of another novel tumor suppressor gene on 5p is possible.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Chromosomes humains de la paire 5
/
Tumeurs colorectales
/
Gènes suppresseurs de tumeur
/
Délétion de segment de chromosome
/
Répétitions microsatellites
/
Perte d'hétérozygotie
/
Allèles
/
Génétique
Limites du sujet:
Adulte
/
Adulte très âgé
/
Aged80
/
Femelle
/
Humains
/
Mâle
langue:
Chinois
Texte intégral:
Chinese Journal of Oncology
Année:
2002
Type:
Article
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